[The structure of oligosaccharide fragments of glycoproteins from influenza virus A/Krasnodar/101/59/(H2N2)--heavy and light chains of hemagglutinin and neuraminidase].
Identifieur interne : 002132 ( Main/Exploration ); précédent : 002131; suivant : 002133[The structure of oligosaccharide fragments of glycoproteins from influenza virus A/Krasnodar/101/59/(H2N2)--heavy and light chains of hemagglutinin and neuraminidase].
Auteurs : N P Arbatski ; A O Zheltova ; D V Iurtov ; V A Derevitskaia ; N K KochetkovSource :
- Bioorganicheskaia khimiia [ 0132-3423 ] ; 1989.
Descripteurs français
- KwdFr :
- Acides aminés (analyse), Chromatographie en phase liquide à haute performance, Glycoprotéines (analyse), Hémagglutinines virales (analyse), Oligosaccharides (analyse), Sialidase (analyse), Sous-type H2N2 du virus de la grippe A, Virus de la grippe A (analyse), Virus de la grippe A (enzymologie), Virus de la grippe A (immunologie).
- MESH :
- analyse : Acides aminés, Glycoprotéines, Hémagglutinines virales, Oligosaccharides, Sialidase, Virus de la grippe A.
- enzymologie : Virus de la grippe A.
- immunologie : Virus de la grippe A.
- Chromatographie en phase liquide à haute performance, Sous-type H2N2 du virus de la grippe A.
English descriptors
- KwdEn :
- Amino Acids (analysis), Chromatography, High Pressure Liquid, Glycoproteins (analysis), Hemagglutinins, Viral (analysis), Influenza A Virus, H2N2 Subtype, Influenza A virus (analysis), Influenza A virus (enzymology), Influenza A virus (immunology), Neuraminidase (analysis), Oligosaccharides (analysis).
- MESH :
- chemical , analysis : Amino Acids, Glycoproteins, Hemagglutinins, Viral, Neuraminidase, Oligosaccharides.
- analysis : Influenza A virus.
- enzymology : Influenza A virus.
- immunology : Influenza A virus.
- Chromatography, High Pressure Liquid, Influenza A Virus, H2N2 Subtype.
Abstract
The structure and heterogeneity of carbohydrate chains of hemagglutinin (HA) and neuraminidase (NA), the surface glycoproteins of influenza virus A/Krasnodar/101/59 (H2N2), were investigated. Hemagglutinin was reduced with beta-mercaptoethanol and its heavy (HA1) and light (HA2) chains were separated by gel chromatography. Amino acid and sugar composition of HA1, HA2 and NA was elucidated. The carbohydrate chains of the glycoproteins were cleaved off by the alkaline LiBH4 treatment and oligosaccharides were reduced with NaB[3H]4. They were fractionated by subsequent two-step HPLC on Ultrasphere-C8 and Zorbax-NH2 columns with simultaneous identification using nonlabelled oligosaccharides of known structures. Some of the major oligosaccharides isolated from HA1, HA2 and NA were thus identified as high mannose chains, containing 5-9 mannose residues, and complex chains, first of all biantennary chains having or not having bisecting N-acetylglucosamine and/or fucose residues. The approach which has been developed enables one to study the structure and heterogeneity of carbohydrate chains starting from one nmole of a desialylated N-glycoprotein.
PubMed: 2634965
Affiliations:
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Le document en format XML
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Kochetkov</name></author></analytic><series><title level="j">Bioorganicheskaia khimiia</title><idno type="ISSN">0132-3423</idno><imprint><date when="1989" type="published">1989</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (analysis)</term><term>Chromatography, High Pressure Liquid</term><term>Glycoproteins (analysis)</term><term>Hemagglutinins, Viral (analysis)</term><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A virus (analysis)</term><term>Influenza A virus (enzymology)</term><term>Influenza A virus (immunology)</term><term>Neuraminidase (analysis)</term><term>Oligosaccharides (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides aminés (analyse)</term><term>Chromatographie en phase liquide à haute performance</term><term>Glycoprotéines (analyse)</term><term>Hémagglutinines virales (analyse)</term><term>Oligosaccharides (analyse)</term><term>Sialidase (analyse)</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Virus de la grippe A (analyse)</term><term>Virus de la grippe A (enzymologie)</term><term>Virus de la grippe A (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Amino Acids</term><term>Glycoproteins</term><term>Hemagglutinins, Viral</term><term>Neuraminidase</term><term>Oligosaccharides</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Acides aminés</term><term>Glycoprotéines</term><term>Hémagglutinines virales</term><term>Oligosaccharides</term><term>Sialidase</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Influenza A Virus, H2N2 Subtype</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term><term>Sous-type H2N2 du virus de la grippe A</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The structure and heterogeneity of carbohydrate chains of hemagglutinin (HA) and neuraminidase (NA), the surface glycoproteins of influenza virus A/Krasnodar/101/59 (H2N2), were investigated. Hemagglutinin was reduced with beta-mercaptoethanol and its heavy (HA1) and light (HA2) chains were separated by gel chromatography. Amino acid and sugar composition of HA1, HA2 and NA was elucidated. The carbohydrate chains of the glycoproteins were cleaved off by the alkaline LiBH4 treatment and oligosaccharides were reduced with NaB[3H]4. They were fractionated by subsequent two-step HPLC on Ultrasphere-C8 and Zorbax-NH2 columns with simultaneous identification using nonlabelled oligosaccharides of known structures. Some of the major oligosaccharides isolated from HA1, HA2 and NA were thus identified as high mannose chains, containing 5-9 mannose residues, and complex chains, first of all biantennary chains having or not having bisecting N-acetylglucosamine and/or fucose residues. The approach which has been developed enables one to study the structure and heterogeneity of carbohydrate chains starting from one nmole of a desialylated N-glycoprotein.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Arbatski, N P" sort="Arbatski, N P" uniqKey="Arbatski N" first="N P" last="Arbatski">N P Arbatski</name><name sortKey="Derevitskaia, V A" sort="Derevitskaia, V A" uniqKey="Derevitskaia V" first="V A" last="Derevitskaia">V A Derevitskaia</name><name sortKey="Iurtov, D V" sort="Iurtov, D V" uniqKey="Iurtov D" first="D V" last="Iurtov">D V Iurtov</name><name sortKey="Kochetkov, N K" sort="Kochetkov, N K" uniqKey="Kochetkov N" first="N K" last="Kochetkov">N K Kochetkov</name><name sortKey="Zheltova, A O" sort="Zheltova, A O" uniqKey="Zheltova A" first="A O" last="Zheltova">A O Zheltova</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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